Friday, July 13, 2012

Application of Three-Dimentional Ultrasound in Obstetrics Dr. N. Malhotra, Dr. J. Malhotra, Dr. Meena, Dr. Vanaj Mathur, Dr. B. Ahuja INTRODUCTION T hree-dimensional (3-D) Ultrasound involves imaging of the distribution of unltrasonic echo information in 3-D space, while conventional ultrasongraphy applies to this imaging on a two-dimensional (2-D) plane. Now 3-D ultrasound is being regarded as the future of ultrasound system after color Doppler system. Voluson 530 D, the only real-time digital 3 D system as well as a very powerful digital CFM, and it shows what the value of future diagnostic oriented system.1 Kretztechnik started in 1974 with the first developments dedicated in 3-D ultrasound. A cylindric-shaped transducer incorporating 25 elements mounted on a drum performed a volume scan consisting of 25 parallel slices. (fig. 1) It was in 1989 in Paris at the french congress of Radiology when Kretztechnik presented the first commercially available ultrasound system featuring the 3 D-voluson technique (voluson- volume sonography). (fig. 2) In 1991, Pretorius and Nelson, discussed the potential advantages of 3 D ultrasound and its usefulness for fetal studies.2 All the studies mentioned above used systems developed by the authors. The most recent development is real-time 3 D ultrasound using a defocusing lens, without computer processing3,4. The 3 D—Voluson Technique Continuous research and development of this technology led to diagnostic applications in various medical fields. The voluson system is based on several main components : • Dedicated voluson transducers providing a fully automatic scan of a user-defined region of the patient’s body. For different applications various transducers ranging from 3.5 — 10 MHZ are available. • A special electronic memory to store the ultrasound data as a geometrically correct volume block . • A digital 3 D scan converter for lossless & fast image processing. Immediately after the volume scan is finished (0.5 to 5 sec.), the monitor displays three orthogonal planes, congitudinal, transverse and coronal planes. (Fig. 3) Each of these planes can be moved within the volume block for detailed analyses, either by parallel shifting (tomographic slicing) or by rotation around any of the three spatial axes. (Fig. 4) Digital 3 D—Sonography Only fully digital technology allows the use of absolutely identical channels. Small deviations in the signal path are enough to produce distortion and noise. Digital technology avoids weakness of this kind and guarantees signal processing which is both looseness and noiseless. This results in superior image quality, high reliability and the stability of the system. Three-Dimensional Ultrasound in Obstetrics Three-dimensional ultrasound with transvaginal scanning in the first trimester. Fetal biometry in the first trimester is often used for the estimation of gestational age. Lasser and co-workers5 examined 144 first-trimester fetuses by transvaginal ultrasonography and showed that gestational age can be estimated much earlier and more accurately than with the transabdominal approach. However, with the use of conventional transvaginal ultrasonography, it is sometimes difficult to obtain an optimal plane for crown-rump measurement for diagnosis. Three-dimensional ultrasound overcomes this problem and increases the accuracy of fetal biometry. (Fig. 5) Three-dimensional ultrasound with transabdominal scanning in and after the late first-trimester with conventional 2 D Ultrasound Scan, a general impression of fetal posture can be obtained, but it can not be described precisely. Surface rendering in 3 D ultrasound overcomes this problem6, 7, 8. Steiner and Colleagues9 determined gestational sac volume in the first trimester by tracing the contour of the gestational sac using transabdominal 3 D ultrasound. In the late second and third trimester, the volume of amniotic fluid decreases relative to fetal size and it becomes increasingly more difficult to obtain 3 D images of the whole body of fetus by surface rendering. Fetal Head Blaas and associates10 obtained 3 D images and calculated the volume of the brain cavities at 7-10 weeks of gestation contours of the brain cavity were interactively drawn in successive 2 D slices and displayed as 3 D images. (Fig. 6) Diagnosis of structural abnormalities such as anencephaly. Encephalocele or choroid plexus cyst can be made by 2 D ultrasound. However, the defects can be better described by displaying orthogonal Triple planes simultaneously.11 (FIg. 7, 8) The corpus collosum, which is difficult to see with 2 D ultrasound, may be depicted with 3 D ultrasound by constructing a section horizontal to the abdominal wall.12 (Fig. 9) Abnormal development of cranial sutures is seen in many dystrophic syndromes and metabolic disturbance. With 2 D ultrasound, curvilinear Cranial structures such as cranial sutures and frontanelles can not be properly evaluated. This is easier with 3 D Ultrasound using a volume rendering method such as the maximum intensity method13 or defocusing lens (real-time 3 D ultrasound)14. (Fig. 10) Fetal face Three-dimensional ultrasound provides clear surface(6, 14, 15, 16, 17, 4, 8) images of the fetal face non-invasively. Images from different directions can also be obtained from 3 D data6, with 3 D ultrasound, the lips18, upper gum18, nose and eyelids can be observed well and morphological anomalies such as single hostric18, flat nose18, proboscis19. Cleft lip17, 18, 19 hypotelorism18 or hypertelorism can be better seen with 3 D ultrasound14, 17, 8 and a low set dysplastic ear can be readily diagnosed17. The facial origin of a fetal teratoma was confirmed on a transparent rational display11. Facial defects are one marker of chromosomal abnormalities20 and 3 D ultrasound may be useful for increasing the selectivity of screening. (Fig. 11, 12, 13, 14) Fetal Skeleton The fetal skeleton can be observed by volume rendering, with techniques such as the transparent method and maximum intensity method11, 15, 16. If the vertebral column is pathologically curved laterally, it is impossible to display the whole vertebral column in one tomogram. Anomalies such as scoliosis17, kyphosis, lordosis and spina bifida may be overlooked by 2 D ultrasound. The advantage of 3 D ultrasound is the ability to visualize both curvatures at the same time. (Fig. 15, 16, 17, 18) Budorick and co-workers21 reviewed the ultrasonography of the fetal spine and pointed out that the curvature of the spine, continuity of vertebral bodies and costovertebral junctions could be observed more clearly with 3 D ultrasound using the depth-cued maximum intensity method, than with 2 D ultrasound. (Fig. 19) The fetal thorax, ribs, vertebrae, clavicles and sternum22 are observed with with 3 D ultrasound, which is useful for diagnosing a small thorax and skeletal dysplasia related to pulmonary hypoplasia. (Fig. 20) Fetal Cardiovascular System In 3 D ultrasound examination of the adult heart with regular rhythm, 3 D data are generally acquired over a period of many heart beating, monitored by an electro cardiogram (ECG). Nelson and colleagues solved this problem by using the movement of a heart wall/valve instead of the ECG, and constructed 3 D images of the fetal heart without distortion due to beating. (Fig. 21) Smith and associates24 developed a 2 D array probe for obtaining 3 D data in real-time and applied it to the fetal heart and real-time 3 D ultrasound with simultaneous multisection display. Fetal Abdomen By surface rendering, abnormalities of the abdominal wall such as omphalocele15, 17 and gastroschisis are well demonstrated. It is possible to construct any slice nearly parallel to mother’s abdominal wall in arbitrary section on orthogonal triple-section display, thus making it to observe the esophageal-gastric junction and pylorus. Three-dimensional ultrasound confirms suspected, multicystic dysplastic kidney as well as renal agencsis and the pelvic-ureter junction12 and ureterovesical junctions are easily observable. Nagata and co-workers25 developed a method for 3 D reconstruction of the fetal stomach mathematically from one ultrasonogram of a longitutinally transected stomach, using the symmetry of the stomach about the central aseis. The inner volume of the stomach can be measured directly from 3 D data. The volume of the bladder is also measurable. Fetal Limbs Surface-rendered images in 3 D ultrasound give clear displays of distortions of the normal anatomical axis such as club foot (talipes) equinovarus)17 and rocker-bottom feet as well as limb abnormalies such as phocomelia26 with 3 D ultrasound, fingers are well observed4, 15. It is thus useful for detecting overlapping fingers6, polydactyly27 and syndactyly. (Fig. 22, 23) With 3 D ultrasound, two orthogonal sections can be displayed together. The section at the exact midpoint of the limb can be obtained with good reproducibility. Favre and colleagues28 determined thigh circumference at the midpoint of the femur and are circumference using 3–D ultrasound and proposed a new formula for fetal weight estimation. Fetal External Genitalia External genitalia can be observed and malformations of the genitalia such as hermaphroditism15 and bipartite scrotum29 can be seen clearly by 3 D ultrasound. (Fig. 24, 25) Estimation of Fetal weight—placental weight and amniotic fluid volume. If the fetal volume can be Measured accurately with 3 D ultrasound, a more accurate fetal weight estimation may be possible. With 3 D ultrasound not-only fetal volume but also placental volume and amniotic fluid volume could be measured in vino. Fetoplacental circulation— Three-dimensional ultrasound shows the distributions of blood flow in the placenta. Thus it facilitates the diagnosis of not only morphological abnormalities but also abnormalities of the distribution of blood flow. (Fig. 26) Limitations of Three-Dimensional Ultrasound 1. The influence of movement of the object on 3 D images, distortion is caused, if fetus or mother moves during the acquisition of 3 D data. So 3 D data acquisition should be carried out—during periods of fetal rest. Merz & co-workers17 state that conventional 2 D ultrasound is superior to 3 D ultrasound for the assessment of cardiac anatomy. 2. Arifacts and other problems in two dimensions also exist in three dimensions. 3. The three-dimensional image is not always useful for diagnosis. In cases of severe obesity or in patients with oligo hydramnios, fetal imaging not only with 2 D ultrasound but with 3 D ultrasound is also difficult. 4. Preprocessing for three-dimensional image construction is time consuming. 5. Scanning range is too narrow, only portions of fetal images can be obtained in the third timester. CONCLUSIONS Three-dimensional ultrasound is very useful diagnostic method in obstetrics. However, 3 D ultrasound is not a substitute for conventional 2 D ultrasound and both methods should be used together to get accurate & efficient ultrasonic diagnosis. 17 Application of Three-Dimentional Ultrasound in Gynecology & Infertility Dr. N. Malhotra, Dr. J. Malhotra, Dr. Meena, Dr. Vanaj Mathur, Dr. B. Ahuja INTRODUCTION T he conventional two-dimentional (2 D) transvaginal ultrasound is a valuable diagnostic tool in the field of gynaecology. Three-dimensional (3 D) ultrasound and reconstruction can investigate more complicated structures and it overcomes some of the limitations of 2 D sonography. The major advantages are—one the ability to obtain ultrasound sections which are impossible to see on a routine scan and the ability to perform accurate volume measurements. Once the volumes are scanned and stored digitally, the images which may be missed easily or visualised incompletely at conventional 2 D sonography can be identified at 3 D ultrasound. In addition, 3 D ultrasound can help in differentiating different uterine malformations and intrauterine pathologic lesions1-3. With the recent advent of 3 D sonography, the diagnosis of the female genital tract pathologies can be achieved accurately. Three perpendicular planes displayed on the screen (Fig. 1) can be rotated simultaneously into a most suitable anatomic orientation from any arbitrary planes. Various imaging modes are available in the processing of 3 D imaging. Volumetry is indicated in the measurement of the tumors or any interested organs. Three-dimentional (3 D) ultrasound has recently been introduced into clinical practice4, 1 in evaluation of the female genital tract. Advantages Despite many recent technical improvement, there are still limitations to the accuracy of ultrasound diagnosis in gynaecology. Patient position and pelvic anatomy severly restrict the movements of the transducer during the scan. This results in a very limited number of scanning planes available for examination. In three-dimensional ultrasonography, the images produced are spatially oriented and allow detailed evaluation in multiple planes. One can examine section by section at any angle, displaying the three orthogonal planes (Fig. 1) concomitantly and increasing diagnostic accuracy & specificity. Volume assessment rather than the 2 D length or circumference measurements are more useful in evaluation of size of an organ or lesion. Distance, circumference or volume measurements are accurate, precise & reproducible with the 3 D ultrasound. The 3 D sonography system from Kretztechnik, Austria enables 0000measurement of tissue brightness using the gray-level histogram5. Use of 3 D ultrasound in routine will increase competence, interest, safety & ease of learning. Three dimensional ultrasound evaluations from various angles & planes makes more easy to understand complex anatomic relationship. It allows an inexperienced person like the patient to understand a mental 3 D impression of the underbying anatomy or pathologic condition without much difficulty. Thus it makes doctor, patient conversations less frustrating & less stressful for both. With increasing demand of long distance communication in real time, 3 D ultrasound may prove very useful. Limitations It in an expensive tool. • 3 D ultrasound should be operated by skilled, talented & experienced sonologist for good results. • Physical barriers like severe obesity, absence of tissue border lines cause the same problems in 3 D as in 2 D imaging. • Patient motion limits quality imaging. • For large masses, high image storage capacity is required to store volume data which adds to the expense. • Real time scanning in 3 D is now available but the frame rates are still slow. Despite of all the above, 3 D imaging in very useful tool as an adjunct to B-mode imaging but it cannot replace B-mode ultrasound. TECHNIQUE OF THREE-DIMENSIONAL SONOGRAPHY OF THE FEMALE GENITAL ORGANS Three-dimensional studies of the female genital tract in our unit are performed using a B-mode scanner which monitors spatial orientation of the images and stores these as a volumes set in the memory of computer (Medison 530 3 D voluson, Kretztechnik, Austria). Conventional & B-mode ultrasound examination is always completed first. Once the position of organ is identified in the longitudinal plane, the ultrasound probe is kept steady & the patient is asked to lie still on the examination bed. The volume mode is then switched on. Three-dimensional ultrasound volume is generated by the automatic rotation of the mechanical transducer through 360. The acquired volume is in the shape of a truncated cone with a depth of 4.3-8.6 cm. & a vertical angle of 90º, using the medium line density, the typical acquisition time is around 10 sec. (Fig. 2 Machine) The obtained volumes may be analyzed immediately or stored and examined later. Computer-generated planar reformatted sections are similar to images obtained by conventional B-mode 2—D sonography. These perpendicular planes are simultaneously shown on the screen thus make easier understanding of pelvic anatomy. The number & orientation of reformatted planes are not limited thus providing multiple sections which could not be obtained on a routine scan. The most useful plane is the transverse section through the whole length of the uterus from the fundus to cervix which cannot be seen on a conventional transvaginal scan. (Fig. 3 : Probes) Kretztechnik, Austria (Medison 530 3 D) utilizes a 3.5/5/0 MHZ transabdominal or a 7.5 MHZ transvaginal volume transducer (Mechanical 3 D probe) to acquire the volume data set. (Fig. 3) We have been mostly interested in the measurement of endometrial volume. Three perpendicular reformatted sections are displayed on the screen and a longitudinal plane is chosen for volume measurements. The actual measurement is performed by delineating the whole of the uterine cavity in a number of parallel longitudinal sections 1-2 mm apart. Then the endometrial volume is calculated automatically by the in-built computer software program. The 3-D reconstructed view can be cine imaged & rotated around an axis at slow or fast speed or slice by slice to a get angle of up to 360º usually, 25 slices of 6-7º Length around a 180º-220º angle from the rotation centre in adequate & may take from 2-5 minutes. The newer machines are becoming ever faster. (Fig. 4 3 D of uterine cavity) CLINICAL APPLICATION OF 3 D ULTRASOUND UTERINE PATHOLOGY (A) Congenital Anomalies Hysterosalpingography (HSG) is widely accepted as a standard method for the diagnosis of congenital uterine anomalies. It is an invasive test which requires the use of contrast medium and exposure to radiation. Although HSG provides a good outline of the uterine cavity, it gives no information about the outer uterine contour. Therefore, the distinction between different types of lateral fusion disorders in impossible.6,7 Definite diagnosis can be made non-invasively with 3 D ultrasound. The cornal/frontal section though the whole length of the uterus is most useful. This plane, being perpendicular to the direction of the ultrasound beam spread, can not be seen on a conventional transvaginal scan. Jurkovic and colleagues2 have suggested that the best time to image the uterus is in the luteal phase when the endometrium is most thickened and echogenic. The fundal cleft and the length of septum can be accurately measured by 3 D ultrasound & allow accurate differential diagnosis (Fig. 5) between an accurate, supseptate and bicornuate uterus. 3 D three-planer image of a normal uterus is having a convex fundus and straight upper line of the uterine cavity. An arcuate uterus has a convex fundus but concave rounded upperline of the uterine cavity. A sepsptate uterus is identified by a septum dividing the proximal portion of the uterine cavity but the uterine fundus is convex or with a shallow cleft (< 1 cm). A bicornuate uterus has two well formed cornua separated by a large fundus indentation2, 80 (> 1 cm) (Fig. 5) An important additional advantage of 3 D ultrasound (in comparison to HSG) is the ability to visualise and obtain clear images of both the uterine cavity & myometrium at the same time without the need for additional invasive testing. The sensitivity, specificity, positive & negative predictive values of 2 D & 3 D ultrasound for the diagnosis of normal uterus & congenital uterine anomalies were calculated in 60 patients with technically acceptable 3 D volumes (Table 1) Table 1 Sensitivity & specificity of two-dimensional (2 D) and Three-dimensional (3 D) ultrasound for the diagnosis of uterine anomalies in comparison with hysterosalpingography. Sensitivity (%) Specificity (%) Normal uterus 2 D 88 94 3 D 98 100 Arcuate uterus 2 D 67 94 3 D 100 100 Major anomaly 2 D 100 95 3 D 100 100 Two-dimensional ultrasound detected all cases of anomalies but there were a number of false-positive findings. The lateral parts of the uterine cavity close to the tubal origin gave a false impression of an arcuate uterus. In these cases a division of the endometrial echo in the lateral uppermost part of the uterine cavity was seen. Due to inability to obtain transverse section through the long axis of the uterine fundus. The distinction between normal & arcuate uterus is often impossible on conventional B-mode scanning. Similarly the diagnosis of different anomalies like subseptate & bicornuate uterine cavity is based on the accurate measurement of the fundal cleft & the length of the Septum. These measurements could not be performed on a traditional scan. The sensitivity of 2 D ultrasound is very high & therefore it can be used as a screening test for congenital uterine anomalies. Three-dimensional ultrasound is used as a diagnostic test in those with a screen positive result on 2 D scan. (B) Leiomyomas & Adenomyosis 3-D ultrasound can be used to asses the volume of uterine myomas or adenomyosis. Serial slices across the lesions are taken for measuring the total tumor volume. Hysterosalpingography can delineate submucous fibroids as filling defects but the exact position of the fibroid is often unclear. 3-D ultrasound can provide accurate diagnosis, localization and measurement of any uterine leiomyoma. Endometrial Pathology Endometrial polyps, submucus fibroids, synechia, carcianoma detection. The feasibility of three-dimensional reconstruction of the endometrium cavity was initially shown by Balen & coworkers, & subsequently by many investigaters.2, 3, 10, 11 Three dimensional scanning provides the more accurate size, depth & orientation of the lesion. In most cases, saline enhancement & 3 D images are essential to assist in specific presumptive diagnosis. (Fig. 6) The technique of 3 D—Saline contrast hysterosonography (3 D—SHSG) has been described by Weinraub’s group.11 After performing 2 D transvaginal ultrasound for screening, a speculum is placed & the vagina cleansed with an antiseptic solution (providone-iodine). A pediatric foley’s cather in inserted into the uterine cavity. The foley’s catheter’s inflated balloon will prevent it from the displacement during 3 D ultrasound examination. Saline must be instilled slowly to obtain a clear view and to avoid discomfort felt by the patient. (Fig. 7) In postmenopausal women with cervical stenosis, a simple insemination catheter can be used to minimize discomfort. The 3 D TVS probe is reinsterted posterior to the catheter, volume images obtained with the probe held still & patient not breathing for approximately 10 seconds. Then the probe can be removed and images stored for evaluation of the endometrial canal. (Fig. 8) Uterine polyps are seen as echogenic protrusions on a stalk, uterine submucus myomas are seen as broad-based protrusion into the endometrial cavity with mixed echogenicity. Uterine synechia appear as irregular multiple echogenic bands. Endometrial cancer is seen as an endophytic intracuminal growth. (Fig. 9, 10, & 11) Bonilla Musoles12 and his coworkers have shown that results of 3 D - SHSG correspond to hysteroscopy as well as histopathological tissue diagnosis. Small submucus myomas & polyps (few millimeters) can be visualized clearly by 3 D ultrasound. Differentiation between intramural and submucus fibroid may help in deciding the treatment modality. Better visualization of Myometral invasion in patients with endometrial cancer was seen with 3 D13 ultrasound. It may allow noninvasive staging of endometrial cancers. (Fig. 12) Endormetrial Cancer Screening Endometrial thickness of less than 5 mm by conventional 2 D TVS is associated with atrophic or normal endometrium in over 96% of cases10,14. However there is significant overlap in endometrial thickness measurements between, hyperplastic, polypoidal and cancerous endometrium. Volume is superior to endometrial thickness in the evaluation of abnormal uterine bleeding and screening for endometrial cancer (Table 2)10. Gruoeck and coworkers10 found significant differences in the mean volume measurements between polyps (2.4 ml. SD 1.86) and hyperplasia (8.O ml, SD 7.81) while the endometrial thickness was not different (15-3 versus 16.0 mm, P > 0.05). Volume of endometrial cancer correlated better with the stage of the cancer and was a significant prognostic factor independent of tumor grade or myometrical invasion. Table—2 Endometrial thickness versus volume in the diagnosis of cancer. Sensitivity Specificity Positive predictive value Endometrial Thickness 15 mm. 83.3% 88.2% 54.5% Endometrial Volume 13 ml 100% 98.5% 91.7% Intrauterine Device Localization Transvaginal sonography has been utilized to determine or verify the location of an intrauterine device (IUD) after insertion or if symtoms of abdominal pain, inability to feel the thread or amenorrhea occur. Two-dimensional ultrasound allows only limited visualization of the IUCD. Lee and colleagues have shown that in 95% cases, the IUCD can be visualized to its full extent by 3 D ultrasound which is impossible to obtain on conventional ultrasound. Incomplete opening of one arm or downward displacement of the IUCD can be easily demonstrated by 3 D ultrasound. (Fig. 13) Ovarian Pathology The ovaries can usually be visualized by transvaginal ultrasound easily. The 3 D ultrasound can further localize the largest dimension of the ovaries in the three planes through rotation of the stored images. Sometimes, it is difficult to localize the ovaries because of their small sizes with no obvious follicles. The detailed search after the documentation of target box scanning with 3 D ultrasound can localize the ovaries. (Fig. 14) Ovarian Cysts/Tumors There is no good clinical or biochemical marker to differentiate between a benign & malignant ovarian cyst. Ultrasound has been used with increasing frequency and multiple scoring system utilizing macroscopic characteristics (papillary projections, septations thick or thin, echogenicity-homogenous or nonhomogenious, consistency-cystic, solid, loculated) to assign a higher risk of malignancy.12, 17 The papillary projection inside the tumor and changes of tumor capsule observed by 3 D scanning facilitates the correct diagnosis of ovarian malignancy. The tumor dimensions, areas & volume can also be calculated with 3 D ultrasound volumetry system. Bonilla-Musoles & colleagues found that it was much easier to study tumor wall regularity/irregularity. They detected 7% more papillary projections with 3 D TVS when compared to 2 D-TVS. In addition, their ability to detect tumor infiltration through the capsule was very impressive & may have significant clinical value in the future. The examination can be completed in seconds but stored data can be analyzed meticulously slice by slice without patient’s presence. Dermoid tumors are difficult to diagnose as they have a variable ultrasound appearance. The 3 D plastic images make easier to differentiate between teeth, bones and areas of calcification & label as benign tumor where as on 2 D only a solid core of increased echogenicity is seen. Bonilla-Musoles and colleagues18 have reported that clots in endometriomas can be better defined on 3 D while they could be mistaken for papillary projection on 2 D. Utero-ovarian adhesions associated with endometriosis can be visualized with 3 D ultrasound, they were able to identify a solid mass as a hemorrhagic corpus luteum cyst rather than a neoplasm. In addition differentiation luteum follicular & corpus between cyst cyst is made simple. Multi Follicular Ovary (MFO) & Polycystic Ovary (PCO) Multiple cystic changes in the ovaries are associated with menstrual irregularity and anovalation. The follicles without increased echogenicity of ovarian stroma are seen in the patients with MFO. However, the cyst in PCO usually demonstrate characteristic peripheral subcapsular follicles. The ovarian and stroma volumes and stromal echogenicity are increased apparently. Therefore, the advantage of 3 D scanning in volumetry is to provide a convenient method to calculate the ovarian volume. 3D ultrasound can identify the characteristics for differential diagnosis between MFO and PCO. (Fig. 15) Ovulation Inductions 3 D ultrasound can accurately measure the true volume of ovarian follicles in follicular development of assisted reproductive therapy (ART) cycles. Kyei-Mensah et al have used 3 D transvaginal ultrasound to scan the ovaries immediately before the procedure of follicular retrieval or assisted reproduction19. The volume of follicular fluid aspirated is more identical with that of 3 D ultrasound, than that of 2 D ultrasound system. Thus, 3 D ultrasound is essential tool for monitoring follicular development during ART cycles. Three-dimensional ultrasound can accurately scan the largest dimensions of the follicles which is critical for the timing of human chorionic gonadotrophin (HCG) injection. Bonilla-Musoles and colleagues18 have shown that the cumulus oophorus can be easily edentified with 3 D TVS. In addition, the imaging of Ovarian hyperstimulation syndrome (OHSS) can also be reconstructed by 3 D transvaginal ultrasound, to see for the exact volume of the enlarged ovaries. Accurate folliculometry is crucial to the safe and effective management of ovulation induction cyeles. and thus improve pregnancy rates. Here 3-D scanning proves to have a definite advantage. Cervix The cervix usually can not be visualized well using the transvaginal approach. It is better to place the transducer in lower vagina without contact with the cervix. Cervical length and the degree of cervical dilation can be clearly measured with the aid of 3 D ultrasound after localization of cervical length. Shortering of cervix and changes of lower uterine segment can be detected early by 3 D ultrasound & thus prevent preterm labor due to cervical incompetence. The application of 3 D ultrasound during the menstrual cycle can also reveal the dilated cervical canal with cervical mucus to confirm the ovulation event. (Fig. 16) Adnexa The fallopian tubes usually can not be visualised with transvaginal ultrasound, even using 3 D ultrasound. They can be seen only in hydrosalpinx or with the presence of cul-de-sac fluid. When there is no intrauterine sac after a period of amenorrhoea by 2 D ultrasound, the small ectopic gestational sac could be noted in the asymtomatic patients as early as possible by using 3 D transvaginal ultrasound20. The senstivity of picking up ectopic gestations will be more by using 3 D. CONCLUSIONS & FUTURE APPLICATIONS Three-dimensional ultrasonography is an exciting and rapidly developing field, providing images of the target organ in multiple tomographic sections. In particular, for the first time, the volume of the target organ can be accurately measured, regardless of its shape. Three-dimensional ultrasound and reconstruction is a safe and noninvasive technique for the assessment of internal genital organs. It shows several advantages over the conventional 2 D scanning. The imaging of 3 D ultrasound allows physicians to understand and appreciate the complex anatomy of the pelvis with ease. However, it is rather time consuming and it needs experience to use 3 D ultrasound sophisticatedly21. Future modifications of 3 D ultrasound in the field of infertility could include a combination with Color Doppler ultrasound to give an index of vascular changes, 3 D real-time ultrasonography and direct quantitative computation of the volume off target. It’s likely that 3 D ultrasound will be accepted in the future as the diagnostic gold standard for the female pelvis


Application of Three-Dimentional
Ultrasound in Obstetrics
Dr. N. Malhotra, Dr. J. Malhotra, Dr. Meena,
Dr. Vanaj Mathur, Dr. B. Ahuja
Introduction
  T
hree-dimensional (3-D) Ultrasound involves imaging of the distribution of unltrasonic echo information in 3-D space, while conventional ultrasongraphy applies to this imaging on a two-dimensional (2-D) plane. Now 3-D ultrasound is being regarded as the future of ultrasound system after color Doppler system. Voluson 530 D, the only real-time digital 3 D system as well as a very powerful digital CFM, and it shows what the value of future diagnostic oriented system.1
      Kretztechnik started in 1974 with the first developments dedicated in 3-D ultrasound. A cylindric-shaped transducer incorporating 25 elements mounted on a drum performed a volume scan consisting of 25 parallel slices. (fig. 1)
      It was in 1989 in Paris at the french congress of Radiology when Kretztechnik presented the first commercially available ultrasound system featuring the 3 D-voluson technique (voluson- volume sonography). (fig. 2)
      In 1991, Pretorius and Nelson, discussed the potential advantages of 3 D ultrasound and its usefulness for fetal studies.2 All the studies mentioned above used systems developed by the authors. The most recent development is real-time 3 D ultrasound using a defocusing lens, without computer processing3,4.
The 3 D—Voluson Technique
      Continuous research and development of this technology led to diagnostic applications in various medical fields. The voluson system is based on several main components :
·                     Dedicated voluson transducers providing a fully automatic scan of a user-defined region of the patient’s body. For different applications various transducers ranging from 3.5 — 10 MHZ are available.
·                     A special electronic memory to store the ultrasound data as a geometrically correct volume block .
·                     A digital 3 D scan converter for lossless & fast image processing.
      Immediately after the volume scan is finished (0.5 to 5 sec.), the monitor displays three orthogonal planes, congitudinal, transverse and coronal planes. (Fig. 3) Each of these planes can be moved within the volume block for  detailed analyses, either by parallel  shifting (tomographic slicing) or by rotation around any of the three spatial axes. (Fig. 4)
Digital 3 D—Sonography
      Only fully digital technology allows the use of absolutely identical channels. Small deviations in the signal path are enough to produce distortion and noise. Digital technology avoids weakness of this kind and guarantees signal processing which is both looseness and noiseless. This results in superior image quality, high reliability and the stability of the system.
Three-Dimensional Ultrasound in Obstetrics
      Three-dimensional ultrasound with transvaginal scanning in the first trimester.
      Fetal biometry in the first trimester is often used for the estimation of gestational age. Lasser and co-workers5 examined 144 first-trimester fetuses by transvaginal ultrasonography and showed that gestational age can be estimated much earlier and more accurately than with the transabdominal approach.
      However, with the use of conventional transvaginal ultrasonography, it is sometimes difficult to obtain an optimal plane for crown-rump measurement for diagnosis. Three-dimensional ultrasound overcomes this problem and increases the accuracy of fetal biometry. (Fig. 5)
      Three-dimensional ultrasound with transabdominal scanning in and after the late first-trimester with conventional 2 D Ultrasound Scan, a general impression of fetal posture can be obtained, but it can not be described precisely. Surface rendering in 3 D ultrasound overcomes this problem6, 7, 8.
      Steiner and Colleagues9 determined gestational sac volume in the first trimester by tracing the contour of the gestational sac using transabdominal 3 D ultrasound. In the late second and third trimester, the volume of amniotic fluid decreases relative to fetal size and it becomes increasingly more difficult to obtain 3 D images of the whole body of fetus by surface rendering.
Fetal Head
      Blaas and associates10 obtained 3 D images and calculated the volume of the brain cavities at 7-10 weeks of gestation contours of the brain cavity were interactively drawn in successive 2 D slices and displayed as 3 D images. (Fig. 6)
      Diagnosis of structural abnormalities such as anencephaly. Encephalocele or choroid plexus cyst can be made by 2 D ultrasound. However, the defects can be better described by displaying orthogonal Triple planes simultaneously.11 (FIg. 7, 8)
      The corpus collosum, which is difficult to see with 2 D ultrasound, may be depicted with 3 D ultrasound by constructing a section horizontal to the abdominal wall.12 (Fig. 9)
      Abnormal development of cranial sutures is seen in many dystrophic syndromes and metabolic disturbance. With 2 D ultrasound, curvilinear Cranial structures such as cranial sutures and frontanelles can not be properly evaluated. This is easier with 3 D Ultrasound using a volume rendering method such as the maximum intensity method13 or defocusing lens (real-time 3 D ultrasound)14. (Fig. 10)
Fetal face
      Three-dimensional ultrasound provides clear surface(6, 14,  15, 16, 17,  4,  8) images of the fetal face non-invasively. Images from different directions can also be obtained from 3 D data6, with 3 D ultrasound, the lips18, upper gum18, nose and eyelids can be observed well and morphological anomalies such as single hostric18, flat nose18, proboscis19. Cleft lip17, 18, 19 hypotelorism18 or hypertelorism can be better seen with 3 D ultrasound14, 17, 8 and a low set dysplastic ear can be readily diagnosed17. The facial origin of a fetal teratoma was confirmed on a transparent rational display11. Facial defects are one marker of chromosomal abnormalities20 and 3 D ultrasound may be useful for increasing  the selectivity of  screening. (Fig. 11, 12, 13, 14)
Fetal Skeleton
      The fetal skeleton can be observed by volume rendering, with techniques such as the transparent method and maximum intensity method11, 15, 16.
      If the vertebral column is pathologically curved laterally, it is impossible to display the whole vertebral  column in one tomogram. Anomalies such as scoliosis17, kyphosis, lordosis and spina bifida may be overlooked by 2 D ultrasound. The advantage of 3 D ultrasound is the ability to visualize both curvatures at the same time. (Fig. 15, 16, 17, 18)
      Budorick and co-workers21 reviewed the ultrasonography of the fetal spine and pointed out that the curvature of the spine, continuity of vertebral bodies and costovertebral junctions could be observed more clearly with 3 D ultrasound using the depth-cued  maximum intensity method, than with 2 D ultrasound. (Fig. 19)
      The fetal thorax, ribs, vertebrae, clavicles and sternum22 are observed with with 3 D  ultrasound, which is useful for diagnosing a small thorax and skeletal dysplasia related to pulmonary hypoplasia. (Fig. 20)
Fetal Cardiovascular System
      In 3 D ultrasound examination of the adult heart with regular rhythm, 3 D data are generally acquired over a period of many heart beating, monitored by an electro cardiogram (ECG). Nelson and colleagues solved this problem by using the movement of a heart wall/valve instead of the ECG, and constructed 3 D images of the fetal heart without distortion due to beating. (Fig. 21)
      Smith and associates24 developed a 2 D array probe for obtaining 3 D data in real-time and applied it to the fetal heart and real-time 3 D ultrasound with simultaneous multisection display.

Fetal Abdomen

      By surface rendering, abnormalities of the abdominal wall such as omphalocele15, 17 and gastroschisis are well demonstrated. It is possible to construct any slice nearly parallel to mother’s abdominal wall in arbitrary section on orthogonal triple-section display, thus making it to observe the esophageal-gastric junction and pylorus.
      Three-dimensional ultrasound confirms suspected, multicystic dysplastic kidney as well as renal agencsis and the pelvic-ureter junction12 and  ureterovesical  junctions are easily observable.
      Nagata and co-workers25 developed a method for 3 D reconstruction of the fetal stomach mathematically from one ultrasonogram of a longitutinally transected stomach, using the symmetry of the stomach about the central aseis. The inner volume of the stomach can be measured directly from 3 D data.
      The volume of the bladder is also measurable.
Fetal Limbs
      Surface-rendered images in 3 D ultrasound give  clear displays of distortions of the normal anatomical axis such as club foot (talipes) equinovarus)17 and rocker-bottom feet as well as limb abnormalies such as phocomelia26 with 3 D ultrasound, fingers are well observed4, 15. It is thus useful for detecting overlapping fingers6, polydactyly27 and syndactyly. (Fig. 22, 23)
      With 3 D ultrasound, two orthogonal sections can be displayed together. The section at the exact midpoint of the limb can be obtained with good reproducibility. Favre and colleagues28 determined thigh circumference at the midpoint of the femur and are circumference using 3–D ultrasound and proposed a new formula for fetal weight estimation.
Fetal External Genitalia
      External genitalia can be observed and malformations of the genitalia such as hermaphroditism15 and bipartite scrotum29 can be seen clearly by 3 D ultrasound. (Fig. 24, 25)
      Estimation of Fetal weight—placental weight and amniotic fluid volume.
      If the fetal volume can be Measured accurately with 3 D ultrasound, a more accurate fetal weight estimation may be possible.
      With 3 D ultrasound not-only fetal volume but also placental volume and amniotic fluid volume could be measured in vino.
      Fetoplacental circulation—
      Three-dimensional ultrasound shows the distributions of blood flow in the placenta. Thus it facilitates the diagnosis of not only morphological abnormalities but also abnormalities of the distribution of blood flow. (Fig. 26)
Limitations of Three-Dimensional Ultrasound
      1.   The influence of movement of the object on 3 D images, distortion is caused, if fetus or mother moves during the  acquisition  of 3 D data. So 3 D data acquisition should be carried out—during periods of fetal rest.  Merz & co-workers17 state that conventional 2 D ultrasound is superior to 3 D ultrasound for the assessment of  cardiac anatomy.
      2.   Arifacts and other problems in two dimensions also exist in three dimensions.
      3.   The three-dimensional image is not always useful for diagnosis. In cases of severe obesity or in patients with oligo hydramnios, fetal imaging not only with 2 D ultrasound but with 3 D ultrasound is also difficult.
      4.   Preprocessing for three-dimensional image construction is time consuming.
      5.   Scanning range is too narrow, only portions of fetal images can be obtained in the third timester.
Conclusions
      Three-dimensional ultrasound is very useful diagnostic method in obstetrics. However, 3 D ultrasound is not a substitute for conventional 2 D ultrasound and both methods should be used together to get accurate & efficient ultrasonic diagnosis.

17
Application of Three-Dimentional
Ultrasound in Gynecology & Infertility
Dr. N. Malhotra, Dr. J. Malhotra, Dr. Meena,
Dr. Vanaj Mathur, Dr. B. Ahuja
Introduction
  T
he conventional two-dimentional (2 D) transvaginal ultrasound is a valuable diagnostic tool in the field of gynaecology. Three-dimensional (3 D) ultrasound and reconstruction can investigate more complicated structures and it overcomes some of the limitations of 2 D sonography. The major advantages are—one the ability to obtain ultrasound sections which are impossible to see on a routine scan and the ability to perform accurate volume measurements. Once the volumes are scanned and stored digitally, the images which may be missed easily or visualised incompletely at conventional 2 D sonography can be identified at 3 D ultrasound. In addition, 3 D ultrasound can help in differentiating different uterine malformations and intrauterine pathologic lesions1-3. With the recent advent of 3 D sonography, the diagnosis of the female genital tract pathologies can be achieved accurately. Three perpendicular planes displayed on the screen (Fig. 1) can be rotated simultaneously into a most suitable anatomic orientation from any arbitrary planes. Various  imaging modes are available in the processing of 3 D imaging. Volumetry is indicated in the measurement of the tumors or any interested organs. Three-dimentional (3 D) ultrasound has recently been introduced into clinical practice4, 1 in  evaluation of  the female  genital  tract.
Advantages
      Despite many recent technical improvement, there are still limitations to the accuracy of ultrasound diagnosis in gynaecology. Patient position and pelvic anatomy severly restrict the movements of the transducer during the scan. This results  in a very limited number of scanning planes available for examination. In three-dimensional ultrasonography, the images produced are spatially oriented and allow detailed evaluation in multiple planes. One can examine section by section at any angle, displaying the three orthogonal planes  (Fig. 1) concomitantly and increasing diagnostic accuracy & specificity.
      Volume assessment  rather than the 2 D length or circumference measurements are more useful in evaluation of size of an organ or lesion. Distance, circumference or volume measurements are accurate, precise & reproducible with the 3 D ultrasound.
      The 3 D sonography system from Kretztechnik, Austria enables 0000measurement of tissue brightness using the gray-level histogram5.
      Use of 3 D ultrasound in routine will increase competence, interest, safety & ease of learning. Three dimensional ultrasound evaluations from various angles & planes makes more easy to understand complex anatomic relationship.
      It allows  an inexperienced person like the patient to understand a mental 3 D impression of the underbying anatomy or pathologic condition without much difficulty. Thus it makes doctor, patient conversations less frustrating & less stressful for both.
      With increasing demand of long distance communication in real time, 3 D ultrasound may prove very useful.
Limitations
      It in an expensive tool.
·                     3 D ultrasound should be operated by skilled, talented & experienced sonologist for good results.
·                     Physical barriers like severe obesity, absence of tissue border  lines cause the same problems in 3 D as in 2 D imaging.
·                     Patient motion limits quality imaging.
·                     For large masses, high image storage capacity is required to store volume data which adds to the expense.
·                     Real time scanning in 3 D is now available but the frame rates are still slow.
      Despite of all the above, 3 D imaging in very useful tool as an adjunct to B-mode imaging but it cannot replace B-mode ultrasound.
Technique of three-dimensional sonography of
the female genital organs
      Three-dimensional studies of the female genital tract in our unit are performed using a B-mode scanner which monitors spatial orientation of the images and stores these as a volumes set in the memory of computer (Medison 530 3 D voluson, Kretztechnik, Austria). Conventional & B-mode ultrasound examination is always completed first. Once the position of organ is identified in the longitudinal plane, the ultrasound probe is kept steady & the patient is asked to lie still on the examination bed. The volume mode is then switched on. Three-dimensional ultrasound volume is generated by the automatic rotation of the mechanical transducer through 360. The acquired volume is in the shape of a truncated cone with a depth of 4.3-8.6 cm. & a vertical angle of 90º, using the medium line density, the typical acquisition time is around 10 sec. (Fig. 2 Machine)
      The obtained volumes may be analyzed immediately or stored and examined later. Computer-generated planar reformatted sections are similar to images obtained by conventional B-mode 2—D sonography. These perpendicular planes are simultaneously shown on the screen thus make easier understanding of pelvic anatomy. The number & orientation of reformatted planes are not limited thus providing multiple sections which could not be obtained on a routine scan. The most useful plane is the transverse section through the whole length of the uterus from the fundus to cervix which cannot be seen on a conventional transvaginal scan. (Fig. 3 : Probes)
      Kretztechnik, Austria (Medison 530 3 D) utilizes a 3.5/5/0 MHZ transabdominal or a 7.5 MHZ transvaginal volume transducer (Mechanical 3 D probe) to acquire the volume data set. (Fig. 3)
      We have been mostly interested in the measurement of endometrial volume. Three perpendicular reformatted sections are displayed on the screen and a longitudinal plane is chosen for volume measurements. The actual measurement is performed by delineating the whole of the uterine cavity in a number of parallel longitudinal sections 1-2 mm apart. Then the endometrial volume is calculated automatically by the in-built computer software program. The 3-D reconstructed view can be cine imaged & rotated around an axis at slow or fast speed or slice by slice to a get angle of up to 360º usually, 25 slices of 6-7º Length around a 180º-220º angle from the rotation centre in adequate & may take from 2-5 minutes. The newer machines are becoming ever faster. (Fig. 4 3 D of uterine cavity)
Clinical Application of 3 D Ultrasound
Uterine Pathology
(A) Congenital Anomalies
      Hysterosalpingography (HSG) is widely accepted as a standard method for the diagnosis of congenital uterine anomalies. It is an invasive test which requires the use of contrast medium and exposure to radiation. Although HSG provides a good outline of the uterine cavity, it gives no information about the outer uterine contour. Therefore, the distinction between different types of lateral fusion disorders in impossible.6,7 Definite diagnosis can be made non-invasively with 3 D ultrasound. The cornal/frontal section though the whole length of the uterus is most useful. This plane, being perpendicular to the direction of the ultrasound beam spread, can not be seen on a conventional  transvaginal scan. Jurkovic and colleagues2 have suggested that the best time to image the uterus is in the luteal phase when the endometrium is most thickened and echogenic. The fundal cleft and the length of septum can be accurately measured by 3 D ultrasound & allow accurate differential diagnosis  (Fig. 5) between an accurate, supseptate and bicornuate uterus. 3 D three-planer image of a normal uterus is having a convex fundus and straight upper line of the uterine cavity. An arcuate uterus has a convex fundus but concave rounded upperline of the uterine cavity. A sepsptate uterus is identified by a septum dividing the proximal portion of the uterine cavity but the uterine fundus is convex or with a shallow cleft (< 1 cm). A bicornuate uterus has two well formed cornua separated by a large fundus indentation2, 80 (> 1 cm) (Fig. 5)
      An important additional advantage of 3 D ultrasound (in comparison to HSG) is the ability to visualise and obtain clear images of both the uterine cavity & myometrium at the same time without the need for additional invasive testing.
      The sensitivity, specificity, positive & negative predictive values of 2 D & 3 D ultrasound for the diagnosis of normal uterus & congenital uterine anomalies were calculated in 60 patients with technically acceptable 3 D volumes (Table 1)

Table 1
Sensitivity & specificity of two-dimensional (2 D) and Three-dimensional (3 D) ultrasound for the diagnosis of uterine anomalies in comparison with hysterosalpingography.
                  Sensitivity (%)                           Specificity (%)              Normal uterus      
                  2 D                                                     88                                 94
                  3 D                                                     98                                100
                  Arcuate uterus
                  2 D                                                     67                                 94
                  3 D                                                    100                               100
                  Major anomaly
                  2 D                                                    100                                95
                  3 D                                                    100                               100
      Two-dimensional ultrasound detected all cases of anomalies but there were a number of false-positive findings. The lateral parts of the uterine cavity close to the tubal origin gave a false impression of an arcuate uterus. In these cases a division of the endometrial echo in the lateral uppermost part of the uterine cavity was seen. Due to inability to obtain transverse section through the long axis of the uterine fundus. The distinction between normal & arcuate uterus is often impossible on conventional B-mode scanning. Similarly the diagnosis of different anomalies like subseptate & bicornuate uterine cavity is based on the accurate measurement of the fundal cleft & the length of the Septum. These measurements could not be performed on a traditional scan. The sensitivity of 2 D ultrasound is very high & therefore it can be used as a screening test for congenital uterine anomalies. Three-dimensional ultrasound is used as a diagnostic test in those with a screen positive result on 2 D scan.
(B) Leiomyomas & Adenomyosis
      3-D ultrasound can be used to asses the volume of uterine myomas or adenomyosis. Serial slices across the lesions are taken for measuring the total tumor volume. Hysterosalpingography can delineate submucous fibroids as filling defects but the exact position of the fibroid is often unclear. 3-D ultrasound can provide accurate diagnosis, localization and measurement of any uterine leiomyoma.
Endometrial Pathology
      Endometrial polyps, submucus fibroids, synechia, carcianoma detection.
      The feasibility of three-dimensional reconstruction of the endometrium cavity was initially shown by Balen & coworkers, & subsequently by many investigaters.2, 3, 10, 11 Three dimensional scanning provides the more accurate size, depth & orientation of the lesion. In most cases, saline  enhancement & 3 D images are essential to assist in specific presumptive diagnosis. (Fig. 6)
      The technique of 3 D—Saline contrast hysterosonography (3 D—SHSG)  has been described by Weinraub’s group.11 After performing 2 D transvaginal ultrasound for screening, a speculum is placed & the vagina cleansed with an antiseptic solution (providone-iodine). A pediatric foley’s cather in inserted into the uterine cavity. The foley’s catheter’s inflated balloon will prevent it from the displacement during 3 D ultrasound examination. Saline must be instilled slowly to obtain a clear view and to avoid discomfort felt by the patient. (Fig. 7)
      In postmenopausal women with cervical stenosis, a simple insemination catheter can be used  to minimize discomfort. The 3 D TVS probe is reinsterted  posterior to the catheter, volume images obtained with the probe held still & patient not breathing for approximately 10 seconds. Then the probe can be removed and images stored for evaluation of the endometrial canal. (Fig. 8)
      Uterine polyps are seen as echogenic protrusions on a stalk, uterine submucus myomas are seen as broad-based protrusion into the endometrial cavity with mixed echogenicity. Uterine synechia appear as irregular multiple echogenic bands. Endometrial cancer is seen as an endophytic intracuminal growth. (Fig. 9, 10, & 11)
      Bonilla Musoles12 and his coworkers have shown that results of 3 D - SHSG correspond to hysteroscopy as well as histopathological tissue diagnosis. Small submucus myomas & polyps (few millimeters) can be visualized clearly by 3 D ultrasound. Differentiation between intramural and submucus fibroid may help in deciding the treatment modality. Better visualization of Myometral invasion in patients with endometrial cancer was seen with 3 D13 ultrasound. It may allow noninvasive staging of endometrial cancers. (Fig. 12)
Endormetrial Cancer Screening
      Endometrial thickness of less than 5 mm by conventional 2 D TVS is associated with atrophic or normal endometrium in over 96% of cases10,14. However there is significant overlap in endometrial thickness measurements between, hyperplastic, polypoidal and cancerous endometrium. Volume is superior to endometrial thickness in the evaluation of abnormal uterine bleeding and screening for endometrial cancer (Table 2)10.
      Gruoeck and coworkers10 found significant differences in the mean volume measurements between polyps (2.4 ml.  SD 1.86) and hyperplasia (8.O ml, SD 7.81) while the endometrial thickness was not different (15-3 versus 16.0 mm, P > 0.05). Volume of endometrial cancer correlated better with the stage of the cancer and was a significant prognostic factor independent of tumor grade or myometrical  invasion.
Table—2
Endometrial thickness versus volume in the diagnosis of cancer.
         Sensitivity                        Specificity     Positive predictive               value
         Endometrial
         Thickness 15 mm.                 83.3%                  88.2%                        54.5%
         Endometrial
         Volume 13 ml                        100%                  98.5%                        91.7%
Intrauterine Device Localization
      Transvaginal sonography has been utilized to determine or verify the location of an intrauterine device (IUD) after insertion or if symtoms of abdominal pain, inability to feel the thread or amenorrhea occur. Two-dimensional ultrasound allows only limited visualization of the IUCD. Lee and colleagues have shown that in 95% cases, the IUCD can be visualized to its full extent by 3 D ultrasound which is impossible to obtain on conventional ultrasound. Incomplete opening of one arm or downward displacement of the IUCD can be easily demonstrated by 3 D ultrasound. (Fig. 13)
Ovarian Pathology
      The ovaries can usually be visualized by transvaginal ultrasound easily. The 3 D ultrasound can further localize the largest dimension of the ovaries in the three planes through rotation of the stored images. Sometimes, it is difficult to localize the ovaries because of their small sizes with no obvious follicles. The detailed search after the documentation of target box scanning with 3 D ultrasound can localize the ovaries. (Fig. 14)
Ovarian Cysts/Tumors
      There is no good clinical or biochemical marker to differentiate between a benign & malignant ovarian cyst. Ultrasound has been used with increasing frequency and multiple scoring system utilizing macroscopic characteristics (papillary projections, septations thick or thin, echogenicity-homogenous or nonhomogenious, consistency-cystic, solid, loculated) to assign a higher risk of malignancy.12, 17
      The papillary projection inside the tumor and changes of tumor capsule observed by 3 D scanning facilitates the correct diagnosis of ovarian malignancy. The tumor dimensions, areas & volume can also be calculated with 3 D ultrasound volumetry system. Bonilla-Musoles & colleagues found that it was much easier to study tumor wall regularity/irregularity. They detected 7% more papillary projections with 3 D TVS when compared to 2 D-TVS. In addition, their ability to detect tumor infiltration through the capsule was very impressive & may have significant clinical value in the future. The examination can be completed in seconds but stored data can be analyzed meticulously slice by slice without patient’s presence. Dermoid tumors are difficult to diagnose as they have a variable ultrasound appearance. The 3 D plastic images make easier to differentiate between teeth, bones and areas of calcification & label as benign tumor where as on 2 D only a solid core of increased echogenicity is seen.
      Bonilla-Musoles and colleagues18 have reported  that clots in endometriomas can be better defined on 3 D while they could be mistaken for papillary projection on 2 D. Utero-ovarian adhesions associated with endometriosis can be visualized with 3 D ultrasound, they were able to identify a solid mass as a hemorrhagic corpus luteum cyst rather than a neoplasm. In addition differentiation luteum follicular & corpus between cyst cyst is made simple.
Multi Follicular Ovary (MFO) & Polycystic Ovary (PCO)
      Multiple cystic changes in the ovaries are associated with menstrual irregularity and anovalation. The follicles without increased echogenicity of ovarian stroma are seen in the patients with MFO. However, the cyst in PCO usually demonstrate characteristic peripheral subcapsular follicles. The ovarian and stroma volumes and stromal echogenicity are increased apparently. Therefore, the advantage of 3 D scanning in volumetry is to provide a convenient method to calculate the ovarian volume. 3D ultrasound can identify the characteristics for differential diagnosis between MFO and PCO. (Fig. 15)

Ovulation Inductions
      3 D ultrasound can accurately measure the true volume of ovarian follicles in follicular development of assisted reproductive therapy (ART) cycles. Kyei-Mensah et al have used 3 D transvaginal ultrasound to scan the ovaries immediately before the procedure of follicular retrieval or assisted reproduction19. The volume of follicular fluid aspirated is more identical with that of 3 D ultrasound, than that of 2 D ultrasound system. Thus, 3 D ultrasound is essential tool for monitoring follicular development during ART cycles. Three-dimensional ultrasound can accurately scan the largest dimensions of the follicles which is critical for the timing of  human chorionic gonadotrophin (HCG) injection. Bonilla-Musoles and colleagues18 have shown that the cumulus oophorus can be easily edentified with 3 D TVS. In addition, the imaging of Ovarian hyperstimulation syndrome (OHSS) can also be reconstructed by 3 D transvaginal ultrasound, to see for the exact volume of the enlarged ovaries.
      Accurate folliculometry is crucial to the safe and effective management of ovulation induction cyeles. and thus improve pregnancy rates. Here 3-D scanning proves to have a definite advantage.
Cervix
      The cervix usually can not be visualized well using the transvaginal approach. It is better to place the transducer in lower vagina without contact with the cervix. Cervical length and the degree of cervical dilation can be clearly measured with the aid of 3 D ultrasound after localization of cervical length. Shortering of cervix and changes of lower uterine segment can be detected early by 3 D ultrasound & thus prevent preterm labor due to cervical incompetence. The application of 3 D ultrasound during the menstrual cycle can also reveal the dilated cervical canal with cervical mucus to confirm the ovulation event. (Fig. 16)
Adnexa
      The fallopian tubes usually can not be visualised with transvaginal ultrasound, even using 3 D ultrasound. They can be seen only in hydrosalpinx or with the presence of cul-de-sac fluid. When there is no intrauterine sac after a period of amenorrhoea by 2 D ultrasound, the small ectopic gestational sac could be noted in the asymtomatic patients as early as possible by using 3 D transvaginal ultrasound20. The senstivity of picking up ectopic gestations will be more by using 3 D.
Conclusions & Future Applications
      Three-dimensional ultrasonography is an exciting and rapidly developing field, providing images of the target organ in multiple tomographic sections. In particular, for the first time, the volume of the target organ can be accurately measured, regardless of its shape. Three-dimensional ultrasound and reconstruction is a safe and noninvasive technique for the assessment of internal genital organs. It shows several advantages over the conventional 2 D scanning. The imaging of 3 D ultrasound allows physicians to understand and appreciate the complex anatomy of the pelvis with ease. However, it is rather time consuming and it needs experience to use 3 D ultrasound sophisticatedly21.
      Future modifications of 3 D ultrasound in the field of infertility could include a combination with Color Doppler ultrasound to give an index of vascular changes, 3 D real-time ultrasonography and direct quantitative computation of the volume off target. It’s likely that 3 D ultrasound will be accepted in the future as the diagnostic gold standard for the female pelvis.


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